Attach oligos to ligands or surfaces

Quick Facts

Availability: DNA or RNA location: 5’, 3’, or internally (shown) Scales: 100 nmol to large scale Purification: HPLC required Symbol for ordering: /5DTPA/, /iDTPA/, /3DTPA/

Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target. Targets are commonly gold, but can also include a variety of fluorescent and nonfluorescent moieties. The Dithiol modifier adds two functionalized thiol (SH) groups, increasing its target binding efficiency when compared to the conventional thiol modifier. An additional dithiol can be added for even greater binding efficiency [1].

Both Thiol- and Dithiol-modified oligonucleotides are shipped in their oxidized (disulfide) states and require reduction by dithiothreitol (DTT) or tris (2-carboxyethyl) phosphine (TCEP) prior to use. A protocol for the reduction of Thiol-modified oligonucleotides is available on the web site under SUPPORT & EDUCATION – SUPPORT – User guides and protocols. Access it here.

To order, select dithiol from the 5’ mods, Internal mods, or 3’ mods tab on the oligo order page. Please note this modification requires HPLC purification.

Other modifications to facilitate your research

See what other modifications are available from IDT. You can find a list of standard modifications we make to oligonucleotides on the Modifications page of our online catalog. And if you don’t find what you are looking for, just send us a request. We often synthesize special, non-catalog requests. Contact us for more information.