Although high-throughput sequencing methods make it possible to sequence the whole genome of an organism in a single instrument run, whole genome sequencing is often neither required nor desired. Factors such as coverage depth needs, experimental focus, sample number, and cost make target enrichment more suitable.
There are two basic approaches to target enrichment, PCR-based amplification and hybrid capture. Either method allows researchers to focus their analysis to specific regions of interest, increasing depth of coverage of targeted sequences and improving the detection of rare genomic events.
IDT offers target capture by in-solution hybridization using xGen® Lockdown® Probes, individually synthesized biotinylated probes that are individually analyzed for quality control before being pooled (if pooling is requested). Being individually synthesized, xGen Lockdown Probes offer great flexibility for target capture. Researchers can order a panel comprising a base number of probes, and then add additional probes based on the outcome of sequencing. If you prefer to test different combinations of probes to achieve the desired sequencing depth for specific genomic regions, you can have your probes delivered individually in plate wells.
A recent, cost-effective addition to our target capture products are xGen Predesigned Gene Capture Pools. These are preconfigured pools of probes targeting the coding sequences (CDS) of human protein-coding genes. Offered at a minimum of 10 genes per order, xGen Predesigned Gene Capture Pools provide you the ability to create custom human gene capture panels for fast delivery. All you need to do is select your genes of interest and choose how you want the probe pools delivered, combined in a single tube or separated in a plate.
xGen Lockdown Panels, listed below, are the perfect choice for studying specific diseases.
These panels offer the most cost-effective solution for sequencing studies of these diseases. For broader discovery applications, IDT offers the xGen Exome Research Panel. This large panel consists of 429,826 individually synthesized and quality controlled xGen Lockdown Probes—covering 19,396 genes and spanning 39 Mb of the human genome. Again, you can add probes for new targets as you make new discoveries or to improve coverage of specific sites. Alternatively, if none of the preconfigured panels meets your research needs, you can create entirely custom panels using xGen Lockdown Probes.
In the following webinar, we discuss the benefits of hybrid capture for targeted sequencing and highlight the ease and flexibility offered by xGen Lockdown Probes and Panels.