Processing
Functional genomics
A mutant A.s. Cas12a (Cpf1) for CRISPR genome editing shows broad temperature tolerance and a high ratio of on:off target activity, with site-to-site reliability rivaling S.p. Cas9
The editing efficiency of wild-type Cas12a (also known as Cpf1) is not as high as Cas9 on average. However, a newly engineered mutant version, Cas12a Ultra, has remarkably improved editing efficiency and works well with hard-to-target PAM sites including many TTTT sites.
