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Express Dual-Labeled Probes

IDT recommends that all probe melting temperature be approximately 6-8 degrees higher than primer melting temperatures. PrimeTime qPCR Probes do not contain Tm enhancing modifications such as Minor Groove Binders (MGB) that are commonly used in assays from Applied Biosystems. Therefore, directly entering of a probe sequence designed using Applied Biosystems software may have a reduced melting temperature within our assay, and performance may be significantly impacted.
PROBE INFORMATION  #1 
 
 
Select your 5' and 3' mods
  Improve qPCR Sensitivity – Use ZEN Double-Quenched Probes. Learn More.
 
 # Bases:
5' - - 3'

Multiple Entry
How many Probes would you like to create?
  
Or
Upload your sequences
Or
Paste your sequences
 

Customized Labels (more detail)
  
Click here to view the Express DLPTM How to Order Guide
   


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Design and Analysis Tools
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