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HDR Donor Oligo Entry
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(5' → 3')
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5' Dye 5' Code 3' Quencher 3' Code Other Show Other 5' Dyes Hide Other 5' Dyes
Base Notations
- RNA, expressed as 'r_', for example rA, rU.
- 2' O-methyl RNA bases are entered as 'm_'.
- Phosphorothioated RNA bases are entered as 'r_*'.
- Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
# Bases:
Include reverse complement
Looking for controls?
NOTE:The same positive and negative controls can be used for both A.s. and L.b. Cas12a (Cpf1) nucleases.
To order, increase the # of items, then copy and paste the appropriate name and sequence from the following list into this ordering page:| Cas12a (Cpf1) positive control crRNAs | |
|---|---|
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Human HPRT |
| Sequence: | GGTTAAAGATGGTTAAATGAT |
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Mouse HPRT |
| Sequence: | GGATGTTAAGAGTCCCTATCT |
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Rat HPRT |
| Sequence: | ATGCTTAAGAGGTATTTGTTA |
| Cas12a (Cpf1) negative control crRNAs | |
|---|---|
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #1 |
| Sequence: | CGTTAATCGCGTATAATACGG |
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #2 |
| Sequence: | CATATTGCGCGTATAGTCGCG |
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #3 |
| Sequence: | GGCGCGTATAGTCGCGCGTAT |
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Batch editing
Edit scale, purification, normalization and services across multiple items simultaneously.Bulk Input
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# Entries:
| Code | Scale |
|---|---|
| Code | Purification |
|---|---|
| Code | Modification |
|---|---|
Processing...
Change All
Include reverse complement
** Change all options will only apply where option is applicable.
Melting Temperature Settings
| Parameter sets | |
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| Target type | |
| Oligo Conc | µM |
| Na+ Conc | mM |
| Mg++ Conc | mM |
| dNTPs Conc | mM |
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