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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What reagents will I need in addition to the Alt-R® RNAs for a CRISPR-Cpf1 genome editing experiment?

The Alt-R® CRISPR-Cpf1 System includes a target-specific CRISPR-Cpf1 crRNA, Cpf1 endonuclease (Alt‑R A.s. Cpf1 Nuclease 2NLS), and carrier DNA (Alt-R Cpf1 Electroporation Enhancer).

Supply your own electroporation reagents to effectively deliver CRISPR reagents into your cell lines. We recommend delivery of an RNP for best results. However, Alt-R CRISPR-Cpf1 crRNA also works well in cells that stably express Acidaminococcus sp. BV3L6 Cpf1 nuclease.

We also highly recommend the use of control crRNAs. Control sequences for the Cpf1 system are provided online and in the user guides, accessible at